Objectives: This study was undertaken to isolate and investigatebacteriological profile and phenotypic signs of food-processing microbiota strains including to Escherichia, Klebsiella, Enterobacter, Staphylococcus, Pseudomonas, Citrobacter and Serratia genera. Strains were isolated during hygienic monitoring twomilk-processing factoriesfor the period from December 2013 to November 2014.
Methods: Swabbingwas done in field studies according food safety management protocols in industryfor enumerated contamination by microorganisms and isolation of strains of microbiota.I dentification of microorganism was done with culture methods followed by PCR verification. Phenotypic features was studied in vitro with standard biochemical and microbiological methods according good laboratory practice.
Results: Bacterial isolates included to Escherichia, Klebsiella, Enterobacter, Citrobacter, Serratia, Staphylococcus and Pseudomonas genera. Gram-positive cocci account for 20.3 % cases of contamination. In 55 (74.3 %) cases, the pathogens belonged to the family Enterobacteriaceae. Isolate investigations included ability to hemolysis, anti-interferon, anti-lysozyme activity, lecithinase activity, ability to form biofilms in a monoculture of microorganisms. Out of 74 isolates 60 (81.1 %) poses variability of morphological and tinctorial signs.
Conclusions: Gram-negative pathogens and more specifically Escherichia coliaccount for a substantial part of bacterial milk-processing microbiota. Most isolates possessed signs of aggression and ability of biofilmsformation. The obtained experimental data will be used for identification of danger factor accordance with the concept of the quantitative analysis of microbiological risk of food productions.
