Jatropha curcas (Linn) or physic nut is a perennial poisonous shrub grows up to 5m high.It belongs to the family Euphobiaceace. The plant originated from Central America but was spread to other tropical and subtropical countries Africa in Sudan it has found.
The oil from the seed is used as biodiesel. The sap from the stem is used to stop bleeding from wound and the plant is also used as fence from animals.
In the present study,leaves jatrophacruces we recollected from Khartoum university faculty of agriculture.
Leaves were washed with distilled water to remove dirt and soil, then dried, and coarsely powdered.
Hundred Grams of the coarsely powdered plant material were exhaustively extracted for four hours with petroleum ether in a Soxhlet apparatus. Petroleum ether ْ extracted was evaporated with a Rota- vapor under reduced pressure. The extract plant material was air-derided, repacked in Soxhelt and was extracted with methanol for six hours. The methanol extracted by the same method but was filtered and evaporated under reduced pressure using Rota vapor. Extract was dissolved in dimethyl sulphoroxide (DMSO) to prepared three concentrations (12.5%, 25%, and 50%).
Aqueous Extract was prepared by adding 50 ml of distilled water to 5 grams of a samples of the coarsely powdered plants materials in conical flask with occasional shaking in water bath (60 C) for 5mints and was then filtered . Three concentrations (12.5%, 25% and 50%). Were made.
The study started by testing the action of extract (petroleum ether ,methanol and Distilled water )of jatropha cruces Leaves on known pathogen fungi (Aspergillus flavus and Candida albicans).Different Concentrations of were put in well set of media (Sabouraud dextrose agar) after sterilization and Inculcated fungi and control (Ketoconazole and Nystattin) after good growth was obtained the test was Read.
At concentration 50% was found (sensitive) to the petroleum etherextract of leaves of Jatropha Curcas.L.Where (25and26) mm of Inhibition zone was recorded.
The concentration of 25% also showed activity to Candida albicans similar to nystatin (20) mm and moderate activity (sensitive) against Aspergillus flavus (17) mm.
Where concentration of 12.5% showed same activity (sensitive) for Candida albicans, (16)mm and inactivity against for Aspergilusflavus.
Methanolic extract of Leaves at the concentrations of 50% , 25% and 12.5% was found inactivity against Candida albicans , but it showed activityagainst Aspergillus flavus where itaninhibtary zone of extract (20)mm of concentration 50% and concentrations 25% and 12.5% was found inactive against Aspergillus flavus. Compared with the control (36) mm. Water extracts of leaves was found inactive against Aspergillus flavus and Candida albicans at all concentrations used.
