Objective: The primary point of the investigation was to screen the silver nanoparticle synthesized from the methanol concentrate of Murraya koenigii for its invitro anticancer activity against MCF 2, PC3, A-549, HeLa and HepG2 Cell lines. Methods: Silver nanoparticles were prepared from methanol concentrate of Murraya koenigii and nanoparticlesynthesised was analysed by UV, SEM and TEM analysis. The impact of nanoparticles synthesized on MCF-2, PC3, A-549, HeLa and HepG2 disease cell lines were assessed by MTT colorimetric assay. After assessing the cytotoxicity effect, the impact of cell cycle and apoptosis was assessed by flow cytometric method. Results: TEM analysis showed the particle size of 25.56 nm for silver nanoparticle. The adequacy of silver nanoparticle synthesised from Murraya koenigii against MCF-2, PC3, A-549, HeLa and HepG2 cell line demonstrated that the hatching of malignancy cells decreased the suitability of PC-3 and A 549 cancer cell lines only with IC50 values as 103.2 and 117.8µg/ml respectively. Cell cycle analysis and apoptosis study showed that Silver nanoparticles are effective in controlling the cell cycle and blocking the apoptosis in all the cell lines used. Conclusion: All in all, Murraya koenigii has critical cell reinforcement movement and anticancer action against all cell lines used.
