Background: Breast Cancer, the most common malignancy causing mortality in women, has its uniqueness in expressing Hormone Receptors - Estrogen and Progesterone Receptors & HER – 2/neu Receptor. These receptors play a key role in prognosis of the disease by affecting the disease progression and deciding the mode of treatment. The aim of this study was to analyze the prevalence of hormone receptor status & HER-2/neu Status in breast cancer. Materials & Methods: In the present study, immunohistochemical assay of 50 tumor block of patients of breast carcinoma was performed to know the ER, PR and HER-2/neu status as well as histological examination. Results: A total of 50 patients were included with a mean age of 48.9 years. The results in present study documented the 64%, 38% and 34% expression rate of Estrogen receptor ( ER), Progesterone Receptor ( PR ) and Human epidermal growth factor receptor – 2 ( HER- 2/ neu ). An inverse correlation of ER with PR & HER-2/neu was observed. A significant association of Tumour size, lymph node involvement was observed with ER, PR & HER-2 / neu expression. Conclusion: In conclusion, the frequency of ER, PR & HER-2/neu Status in patients with breast cancer is almost same as reported in the literature. Hormone receptor and HER-2/neu receptor status are in inverse relationship. These findings have important implications for ensuring appropriate choices of treatment.