Protective effect of senna tora, bosciasenegalensis, hibiscus sabdariffa, dichrostachys glomerata, adansonia digitata and balanites aegyptiaca extracts against ccl4 induced oxidative stress in rats

Objective: The aim of this study was to investigate the ability of extracts of Senna tora, Bosciasenegalensis, Hibiscus sabdariffa, Dichrostachysglomerata, Adansoniadigitata and Balanitesaegyptiaca to protect and improve the alterations causedbyCCL4 in rats.
Material and methods: Aqueous and ethanolic extracts of these different plants were analyzed for total phenolic, total flavonoid content and in vitro antioxidant activity (DPPH and ABTSradical scavenging activity, total antioxidant capacity). For in vivo antioxidant activity, fourteen adult Wistar albino rats weighing 200-250 g were used divided into eight groups with each group constituted of 5 rats. For induction of oxidative stress, CCl4 was mixed with oliveoil by a 1% ratio, then was directly injected intraperitoneally (1 mL/kg/body weight). The Positive control group was fed with astandard diet during the experiment, negative control group was fed with standard diet for seven days and received a single dose of CCl4 solution intraperitoneally(1mL/kg), the rest of the groups were fed with a standard diet and were given the ethanolic extracts of each of the plants orallyata dose of250mg/kg for seven days and received CCl4(1 mL/kg) solution intraperitoneally on the eighth day. At the end ofthe experiment, lipid peroxidation, catalase and superoxide dismutase were evaluated.
Results: Rats which consumedethanolic extracts of the plants expressed the highest content of total phenols and flavonoids and lowest IC50 for DPPH and ABTS. Globally rats pretreated with ethanolic extracts of the plants presented asignificant reduction (p<0.05) of MDA levels compared to those of the negative control group. Pretreatment of rats with extracts of plants improvedenzymatic activities of catalase and superoxide dismutase. Fruits of D. glomerata exhibited the most significant positive effect.
Conclusion: The results of the present study showed that ethanolic extract of these different plants have antioxidant and scavenging activities as they ameliorated the effects produced by CCl4 in the experimental rats. Therefore, they could be used for the management of diseases associated with oxidative stress like cardiovascular diseases.