Species of Indigofera (Leguminosae) genus used as traditional medicines in different parts of the world, some of them are not studied for its chemical diversity. The present study was aimed to characterize the phytochemical potential of Indigofera Viscosa leaf. Nonpolar to polar solvents were used to successively extract the phytochemicals by using soxhlet apparatus. The extracts were tested for the secondary metabolite screening. Major bioactive compounds such as alkaloids, flavonoids, tannins, steroids, triterpenoids were detected from various extracts. Ethanol extract showed high positive results in the test of such biochemicals, so this extract only used for further study. High amount of total phenolic content was noted in the quantification of secondary metabolites. FTIR analysis indicated the presence of metabolically active alcohols, phenols, carboxylic acids, amino acids, alkynes, esters, nitro compounds, aromatic amines, etc. GC-MS analysis revealed that presence of 15 major compounds in leaf, most of them are well-known for their biological activities. The compound milbemycin B may highly present in the leaf sample because of its large area percentage.The present study will be helpful to the quality assessment of herbal remedies containing Indigofera species and chemotaxonomic justification of the species. Further investigations are required to study the biological activities of the plant in crude as well as elute form of extracts.
