Neutrophil granulocytes and platelets are key players in wound healing and in combination with their secreted growth factors/cytokines can facilitate tissue regeneration and the formation of new blood vessels (angiogenesis). In a recent Kitamura Y. et al. Study, they showed a method for direct estimation of platelet counts in the PRF. These authors used a recombinant t-PA, through a digestion method. They showed that t-PA is powerful enough to be able to count the dispersed platelets aggregated in insoluble fibrin matrices enriched with platelets. Authors have elaborated the data obtained in the figures and tables provided, related to the work published by the Niigata University group, and have evaluated the percentage deviation between "counts with t-PA" and PLT and WBC values with "counts for subtraction "and with" counts for blood count "with the use of statistical techniques. By reducing the value of leukocytes in the PRF obtained by the subtraction method by 34.35%, the value reported with the t-PA method is obtained; reducing the value of the platelets in the PRF membrane obtained from the same method by 19.69%, the value reported with the t-PA method is always obtained; instead of reducing the value of leukocytes obtained from the blood count by 34.12%, the value reported with the t-PA method is obtained and reducing the value of the platelets obtained with the same method by 15.12%, the value reported with the method is obtained from digestion with t-PA. With this experiment the Authors made it possible to validate a simple and inexpensive system for calculating the precise number of platelets and leukocytes present in second-generation platelet concentrates, making it easy to evaluate individual PRF matrices in a timely manner in a clinical setting. It is also possible, with the same method, to evaluate the minimum/maximum level of platelets and leukocytes useful for having a clinical result in the use of Second Generation Platelet Concentrates.
