Background and objective: Pain may also cause detrimental effects on child’s future abilities to learn and remember new information. The most common painful procedures performed during infancy are routine injections without pain management. Non pharmacological and pharmacological methods should be considered for pain relief by health staffs. So the current study was carried out to assess the effect of facilitated tucking on pain during Hepatitis B vaccination among newborns. Methodology: A randomized controlled trail was used. The target population were the term neonates who underwent hepatitis B vaccination. Facilitated tucking position was provided prior to vaccination (n=21) were compared with those of non-tucking (n=21).The outcome measured were the pain score using Neonatal Infant Pain Scale (NIPS) and physiological parameters. Result: There was no significant difference in the pain level between control (4.47 1.16) and treatment group (3.76 0.94) during vaccination, whereas there was a significant difference in the level of pain 3 minutes after vaccination (p<0.05) and also there was a significant difference in respiratory rate between the group during vaccination (p<0.05). Conclusion: Facilitated tucking is found effective in reducing the pain during Hepatitis B vaccination among the term newborns. Hence facilitated tucking position can be used as a non-pharmacological method during painful procedure, as this position make the infant comfortable, more secure with controlled response and it is a simple, inexpensive and non-invasiveintervention.