A comparative study of antimicrobial efficacy of nisin, guduchi, chlorhexidine gel, and calcium hydroxideas intracanal medicament against enterococcus faecalis

Aim: The aim of the study is to compare the antimicrobial efficacy of chlorhexidine gel, calcium hydroxide, Nisin and Guduchi against Enterococcus faecalis.
Methods: Sixty single rooted sixty lower premolar teeth were collected which were extracted for orthodontic purposes. Sixty samples were segregated into four groups with 15 samples in each: Group I: Guduchi extract, Group II: nisin, Group III: chlorhexidine gel, and Group IV: calcium hydroxide.Access cavity preparation was done. Cleaning and shaping were done using Protaper universal file till F3. Decoronation of the teeth was done with diamond saw to obtain a standardized root length of 14 mm, and the apical end from outside was sealed using paraffin wax. under standard conditions teeth were autoclaved. After autoclaving, Phosphate-buffered saline solution was introduced into the roots followed by incubation for 24 h to check for the disinfection of roots. After the disinfection of roots, inoculation of E. faecaliswas done and the apex was sealed. Following bacterial colonization inside the roots, antimicrobials were introduced. Antibacterial efficacy was evaluated for these different antimicrobials. Statistical analysis was done
Results: Significant antibacterial effect against E. faecalis was observed with chlorhexidine gel followed nisin, calcium hydroxide Guduchi
Conclusion: Within studies limitation, it can be concluded that chlorhexidine gel followed by nisin had better antimicrobial properties against E. faecalis than other medicaments