VANCOMYCIN-RESISTANT ENTEROCOCCI SEQUENCE TYPES PREVALENT IN HOSPITALS IN TRINIDAD AND TOBAGO

Author: 
Akpaka, PE*., Kissoon, S., Jayaratne, P and Golding, GR

Vancomycin-Resistant Enterococci (VRE) infections are serious healthcare problem worldwide. VRE
sequence types prevalent in hospitals in Trinidad & Tobago were delineated after analyzing 45 VRE
isolates (E. faecium n=38; E. faecalis n=7) by multiplex polymerase chain reaction (PCR), pulsedfield
gel electrophoresis (PFGE) and multilocus sequence types techniques. All isolates possessed esp
and vanA and B genes but no hyl genes. PFGE revealed five clones among the E. faecium and one
clone among the E. faecalis suggesting that there has been a horizontal spread or intra/inter hospital
spread of the clones at various geographic locations in the country. Sequence types - ST412 (69%),
ST736 (23%) and ST203 (8%) were observed. The predominant VRE sequence type in the country is
ST412 and belong to clonal complex 17 (CC17) similar to ones observed in the Americas. Since these
are associated with nosocomial infections outbreaks, continuous surveillance and infection control
measures must remain a priority in hospitals and health care facilities in Trinidad and Tobago in order
to prevent any potential of outbreaks by this VRE clonal complex.

Page: 
1150-1155
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