The study is an effort to identify correlation between the levels of creatine kinase-MB (CK-MB) with birth asphyxia in term babies and to ascertain whether this enzyme is helpful in determining the severity and prognosis of birth asphyxia. A serum CK-MB value of >92.6 IU/L at 8± 2 hour of life and > 39.2 IU/L at 72 ± 2 hour of life was taken as cut off level. The mean CK-MB level among the case group is 251.67 ± 251.15 and 164.27 ± 211.22 at 8± 2 hour of life and 72± 2 hour of life respectively and among the control group is 48.99 ± 19.51 and 33.9 ± 12.26 at 8± 2 hour of life and 72± 2 hour of life respectively. Estimation of CK-MB can help in distinguishing an asphyxiated from non-asphyxiated neonate helping in management of these babies.