The ancient ayurvedic text Astangahridaya of Vagbhata during seventh century A.D., prescribes a specific formulation comprising four herbs namely Solanum surratense, Zingiber officinale, Tinospora cordifolia and Piper longum for antipyretic and analgesic medication. To validate the ayurvedic uses of this formulation, antipyretic and analgesic efficacy study was performed following acute toxicity study. Physiochemical, pharmacognostical, phytochemical screenings including HPTLC chromatogram were performed for proper standardization of the formulation. The antipyretic effect was assessed using yeast induced pyrexia method. Hot plate, tail-flick and writhing test were used for determining the analgesic properties. Phytochemical analysis revealed the presence of phenols, flavonoids, alkaloids, tannin etc. Significant antipyretic (P<0.001) and analgesic (P<0.01) properties were noticed in dose dependent manner after aqueous extract administration especially at the dose of 500 mg/kg in rodents. Activities of the test drug were sustained and significantly comparable to the standard drugs. Test drug possesses significantly high antipyretic and analgesic properties without any acute toxicity possibly due to presence of flavonoidic phenolic compounds.
