Blagovesta Pencheva ., Rossen Mihaylov, and Dilyana Stoeva

Background: Helicobacter Pylori(HP) diagnostics requires use of invasive and non-invasive
methods. The endoscopy is considered the "gold standard", but it is expensive and difficult to accept
by patients. From non-invasive methods, the most widely used is determination of HP antigens in
faeces and antibodies in blood serum. We used these two tests due to low cost and ease of acceptance
by the individuals.
Methods: The concentration of IgG in the blood serum is determined with Anti-Helycobacter pylori
ELISA (IgG) (Euroimune).Antigens of HP are determined in faeces by enzyme-linked
immunosorbent assay (ELISA).
Results: The percentage of positive results for antigens and antibodies is slightly higher in men
compared to women (40% to 36% and 25% to 20%). Furthermore, the test for antigens in faeces
shows higher sensitivity (40% and 25%) than that for antibodies (36% and 20%). When combining
both tests the sensitivity is increased in men and women by 11%.
Conclusions: The highest is the percentage of detection of HP in combining the two tests(antigens
and antibodies) and the lowest is in that for antibodies. Our results demonstrate a relatively high
frequency of positive results for antigens and antibodies in our country. of HP and even higher
frequency in the simultaneous use of both tests.

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