Evaluation of the chemical composition and antibacterial effects of the viola odorata linn oil's

Azadeh Foroughi, Pouya Pournaghi, Reza Tahvilian, Mohammad Mahdi Zangeneh, Akram Zangeneh and Rohalah Moradi.

Increasing microbial resistance to chemical antibiotics and their probabilistic side effects cause
popularity of medicinal plants, so there is an instantaneous and steady need for novel antimicrobial
compounds from plants. As we know, there is no documented proof on antibacterial effects of Viola
odorataLinn (VOL) oil in west of Iran. The aim of the study was to evaluate the chemical
composition and antibacterial activities of the oil from VOL against Escherchia coli O157:H7 and
Staphylococcus aureus. Gaschromatography mass spectrometry was run to specify their chemical
composition. As a screen test to detect antibacterial properties of the oil, agar disk and agar well
diffusion methods were employed. Macro broth tube test was performed to determinate MIC.
Presence of Pentane 2, 3, 4- Trimethyl, N- Hexadecanoic acid, 10- Undecyn-1-ol and Pentadecanoic
acid were discovered in composition of the obtained oil of VOL Also, The MIC and MBC values
were 0.031 g/ml for the oil in both of tested bacteria. Thus, the research represents the antibacterial
effects of the medical herb on E. coli and S. aureus. We believe that the article provide support to the
antibacterial properties of the oil. The results indicate the fact that the oil of the VOL can be useful as
medicinal or preservatives composition.

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