Effect of diode laser on e.faecalis infected root canals in comparison to sodium hypochlorite- an in vitro study

Ashwini Gaikwad and Varsha Pandit

The aim of the study was to evaluate the effect of Diode Laser on E.Faecalis infected root canals in
comparison to sodium hypochlorite. After decoronation and preparation of the root canals, they were
sterilised. A liquid culture suspension of 0.5 Mcfarland standard BHI broth was prepared to obtain
1.5× 10⁸ colony forming units/ml from a subculture of E.faecalis. Sterilised teeth were kept in an
Eppendrof tube of the bacterial suspension and was incubated for a week under aerobic and static
conditions at 37°C.The medium was confirmed for E.faecalis growth. The cultures were checked for
purity by Gram stain and colony morphology on BHI agar with 10% sheep blood. In the Laminar
Flow Chamber, Group A samples were irrigated with 2.5% sodium hypochlorite and Group B
samples were treated with diode laser. After 10 fold serial dilutions, aliquots of 0.1 ml were plated
onto BHI agar plates and incubated at 37°C for 24hr.The colony forming units grown were counted
and recorded. Results were calculated by measuring the zones of inhibition. Diode laser and Sodium
hypochlorite were proved to be equally efficacious.

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