A new simple, specific, precise and accurate revere phase liquid chromatography method has been developed for estimation of Metaprolol tartrate single dosage forms. The chromatographic separation was achieved on a 5 – micron C 18 column (250x 4.6mm) using a mobile phase consisting of a mixture of Orthophosphoric acid buffer: Acetonitrile (75:25) was used pH 2.0 The flow rate was maintained at 1.0 ml / min. The detection of the constituents was done using UV detector at 254 nm for Metaprolol tartrate. The retention time of Metaprolol tartrate found is 3.84 min respectively. The developed method was validated for accuracy, linearity, precision, limit of detection (LOD) and limit of quantification (LOQ) and robustness as per the ICH guidelines.
