Aim To compare the Widal and dot-EIA with the blood culture in the diagnosis of Typhoid Fever. Methodology: Clinically suspected typhoid fever of aged 1 to 12 year are included. Case details & appropriate investigations are done & results are analysed. Results total of 140 children were screened for enteric fever, out of which 30 cases positive for Widal, 39 Dot-EIA & 20 for blood cultures. Conclusion dot-EIA have a higher sensitivity compared to Widal test. Dot-EIA could be a practical alternative to Widal test even in the resource constrained laboratories.