Urinary Tract Infection is a common, medical complication of pregnancy. UTl is of great clinical concern in this group, as it has adverse maternal and obstetric outcomes. The changing pattern of antimicrobial resistance of uropathogen is a growing problems. Knowledge about the current trends of bacterial profile and their antibiogram pattern causing Urinary tract infection in pregnant women may help clinically to choose the correct empirical treatment. The present study was carried out to determine the trends in antimicrobial susceptibility of uropathogen in pregnant women having Urinary tract infection. A total of 50 urine samples collection for Urinary tract infection in pregnant women, during July 2016 to January 2017 and these were processed in the laboratory. Out of 50 samples analyzed 35 samples were found to contain significant bacteria. Result from the study revealed that the prevalence of Urinary tract infection was more in first trimester (14%) followed by third trimester (12%) and least prevalence was observed in the second trimester was higher in age group of (20 - 24) years. Among 35 isolates obtained from 50 samples, majority of isolates were Gram positive bacteria (71.4%) whereas Gram negative organisms was found to be (28.57%). In UTl, four different uropathogen were identified as E.coli (54.14%) the most predominant uropathogen, followed by S.aureus (28.57%), Proteus vulgaris (8.57%) respectively. The least prevalent bacteria isolated was Klebsiella pneumonia (5.71%). The most surprising result was obtained in case of all the isolates showing resistance to all ten different type of antibiotic indicating multi- drug resistance of uropathogen.