Antioxidant potential, antidepressant activity and hptlc fingerprinting of phenol & flavonoid compounds of cleome viscosa seed extracts

Aaditya Singh., Shalini Tripathi and Singh P. N

Objective: The present study is design to evaluate preliminary phytochemical constituents, physiochemical evaluation, free radical scavenging activity, HPTLC analysis and Antidepressant activity of Cleome Viscosa seed oil.
Methods: Total phenol content, Total flavonoid content, Total tannin content, DPPH radical scavenging method, HPTLC analysis for phenols and flavonoids compounds, Tail suspension test(TST) and Forced swimming test (FST).
Result: Phytoconstituents like alkaloids, flavonoids, saponins, fixed oil etc were assessed by preliminary phytochemical screening. Oil contains an acid value, saponification value, iodine value of 36.57 mg KOH/g, 257 mg KOH/g and 117.1 mgI2/100g respectively. Phenol content, flavonoid content and tannin content is 63.36 ± 0.32 mg gallic acid equivalent/g, 153.47 ± 0.56 mg rutin equivalent/g, 19.4 ± 0.40 mg tannic acid equivalent/g respectively and also having a very good radical scavenging activity. The HPTLC analysis assessed that, the methanolic extract has phenol and flavonoid compounds and also possess significant antidepressant activity.
Conclusion: Our results suggest that Cleome Viscosa seeds are may be proved to be a natural antioxidant and antidepressant with various bioactive compounds used for the treatment of various other diseases.

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