Coumarins are a group of plant-derived poly¬phenolic compound, serve as antimutagenic, anti-proliferation and anti-carcinogenesis in various in vitro and in vivo models. A polyphenolic acetate i.e. Ellagic Acid Peracetate (EAPA) has been known as an antimutagenic that prevents AFB1-evoked genotoxicity in bone marrow and lung cells in vivo model. In this study Ellagic Acid Peracetate (EAPA) was examined for its anticancer activity in Non-Small Cell Lung Cancer cells. To determine the mechanism of its action, cell viability, cell cycle arrest, apoptosis and expression of p21 and NF-ҡB genes were analysed by MTT, flow cytometric and real-time PCR (RT-PCR) assays. EAPA treatment causes decreased viability of A549 cells in a dose as well as time-dependent manner and triggered apoptosis and cell cycle arrest by flow cytometric assayed in A549 cells. EAPA also up regulated p21 and NF-ҡB genes as analysed by real-time PCR (RT-PCR) assay and suggest implication of these genes in the underlying mechanism of EAPA-elicited apoptosis and cell cycle inhibition in A549 cells. Our data supports EAPA to be an efficacious cytotoxic drug in lung adenocarcinoma treatment.
