Antibacterial activity of lactobacilli against extended spectrum β-lactamase producing isolates from post-operative urinary tract infections in cardiac patients

Samyyia Abrar, Noor Ul Ain, Fizza Fatima, Hassnain Sajjad, Shahida Hussain, Muhammad Saeed and Saba Riaz

Purpose: Lactobacilli (LAB) can serve as better supportive supplements in boosting the immune response and act as remedy against life threatening infections. Isolation and characterization of LAB against Extended Spectrum β-Lactamase (ESBL) producing bacteria isolated from postoperative urinary tract infections of cardiac patients.

Methods: ESBL producing isolates were characterized by using phenotypic tests including: combination disc (CD) and double disc synergy test (DDS) and PCR based detection tests. Lactobacilli were isolated from commercial yogurt and milk samples. Characterization was done by using biochemical battery. Well Diffusion assay was used to check antibacterial activity.

Results: Test organisms were obtained from study conducted in Punjab Institute of Cardiology (PIC), Lahore, Pakistan from Oct-2016 to Feb-2017. ESBLs producing strains were isolated from post-operative urinary tract infections (UTIs) in cardiac patients. Fourteen strains were selected as potential probiotics as they possessed strong antibacterial activity against ESBLs. These strains showed variable zone of inhibition, Heat, proteinase k and Sodium dodecyl sulphate (SDS) treatments showed that the antibacterial compound is protein in nature.

Conclusion: Lactobacilli isolated from dairy products may be used against ESBLs. Isolated strains were resistant to low pH and were able to inhibit the growth of ESBLs.

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