The contaminated injection equipment have pivotal role in spread of HCV infection. IV drug users are one of the populations with high risk of HCV infection. Objectives: our goal was to evaluate the role of a hazardous action (intravenous injection with contaminated equipments) in threat of public health. In this study, we collected data on 37 psychiatric patients with a history of IV drug abuse and addiction with HCV and compared to psychiatric patients with a history of IV drug abuse without HCV. Here was measured biochemical and hematological parameters and analyzed by SPSS. We found levels of AST and ALT significantly increased, but ALP and albumin none significantly decreased in patients (p≤0.05). Moreover, PT, aPTT and ESR levels in patients were slightly higher than control group. In addition, a significant correlation between AST and ALT to serum creatinine in patients may indicatea relationship between kidney problems followed by hepatic damage. The mean value of FBS, Tg and cholesterol levels were nearly similar in both groups. The serum level of calcium and phosphate in patients were significantly lower and higher than control group respectively (p≤0.05). There was a reduction and increment in serum level of free T3 and free T4 respectively and the level of TSH in patients was nearly 3 times higher than control group. Thus, primary hypothyroidism has been seemedat patients. Finally, we concluded the evaluation of IV drug abuser for prognosis of HCV widespread is very important.